Western blot analysis of extracts of various cell lines, using Nitro-Tyrosine antibody (TA379138) at 1:1000 dilution.HeLa and C6 cells were treated by Perxynitrate（3mM）for 1 hours.NIH3T3 cells were treated by Perxynitrate（3mM）for 3 hours.Jurkat cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.