Western blot analysis of extracts of various cell lines, using Phospho-HSL-S563 antibody (TA378066) at 1:1000 dilution.3T3L1 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.H9C2 cells were treated with Isoproterenol (10 μM for 20 min) at 37℃.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.