Western blot analysis of extracts of Rat lung, using Sonic Hedgehog (Shh) antibody (TA381477) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.

Immunofluorescence analysis of HeLa cells using Sonic Hedgehog (Sonic Hedgehog (Shh)) Polyclonal Antibody (TA381477) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using Sonic Hedgehog (Sonic Hedgehog (Shh)) Polyclonal Antibody (TA381477) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.