Western blot analysis of extracts of various cell lines, using Phospho-c-Jun-T91 antibody (TA377672) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37‚ÑÉ for 30 minutes after serum-starvation overnight.Both 293T cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.