Western blot analysis of extracts from normal (control) and AMPKα1 knockout (KO) HeLa cells, using AMPKα1 antibody (TA380325) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 3min.