Western blot analysis of extracts of various cell lines, using Phospho-AMPKa1/AMPKa2-T183/T172 antibody (TA380332) at 1:1000 dilution.293T cells were treated by oligomycin (0.5 uM) at 37‚ÑÉ for 30 minutes.C2C12 cells were treated by oligomycin (0.5 uM) at 37‚ÑÉ for 30 minutes.C6 cells were treated by oligomycin (0.5 uM) at 37‚ÑÉ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit .|Exposure time: 60s.