Western blot analysis of extracts of NIH/3T3 cells, using Phospho-EPHA2-S897 antibody (TA375928) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS at 37‚ÑÉ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 1s.