Western blot analysis of extracts of U937 cells, using Phospho-LCP2-Y145 antibody (TA377975) at 1:1000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 10s.