Western blot analysis of extracts of various cell lines, using UBE2O antibody (TA383024) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

Immunofluorescence analysis of HeLa cells using UBE2O Polyclonal Antibody (TA383024) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of A-431 cells using 3ug UBE2O antibody (TA383024). Western blot was performed from the immunoprecipitate using UBE2O antibody (TA383024) at a dilition of 1:1000.