Western blot analysis of extracts of Mouse brain, using Phospho-Tau-Thr212/Ser214 antibody (TA378339) at 1:1000 dilution.Mouse brain were treated by CIP(20uL/400ul) at 37‚ÑÉ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.