Western blot analysis of extracts of HeLa and HT-29 cells, using Phospho-AURKA-T288 antibody (TA373864) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) for 20 hours.HeLa cells were treated by Nocodazole (50ng/mL) for 20 hours.HT-29 cells were treated by Hydroxyurea (4 mM) for 20 hours or treated by Nocodazole (100ng/mL) for 16 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 90s.