Western blot analysis of extracts of various cell lines, using Phospho-ULK1-S467 antibody (TA383096) at 1:1000 dilution.HeLa and Jurkat cells were treated by Serum-starvation overnight at 37‚ÑÉ.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.