Western blot analysis of extracts of various cell lines, using S100A6 antibody (TA381218) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 60s.

Immunoprecipitation analysis of 200ug extracts of U-251MG cells using 3ug S100A6 antibody (TA381218). Western blot was performed from the immunoprecipitate using S100A6 antibody (TA381218) at a dilition of 1:1000.