Western blot analysis of extracts of various cell lines, using MEK2 antibody (TA378249) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

Immunofluorescence analysis of NIH/3T3 cells using MEK2 antibody (TA378249) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using MEK2 antibody (TA378249) at dilution of 1:100. Blue: DAPI for nuclear staining.