Western blot analysis of extracts of HeLa cells, using Phospho-IκBα-S36 pAb (TA379099) at 1:1000 dilution or IKB alpha antibody (A11397).HeLa cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 30s.