Western blot analysis of extracts of C6 cells, using Phospho-β-Catenin-S675 antibody (TA375204) at 1:1000 dilution. C6 cell lysates were treated by CIP at 37°C for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit .|Exposure time: 180s.