Western blot analysis of extracts of Jurkat cells, using Phospho-SMO-S611/615 antibody (TA381789) at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37‚ÑÉ for 30 minutes.Jurkat cells were treated by Anisomycin (25 ug/mL) at 37‚ÑÉ for 20 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 60s.