Western blot analysis of extracts of HeLa cells, using Phospho-TrkA-Y674/675 + TrkB-Y706/707 antibody (TA379372) at 1:500 dilution.HeLa cells were treated by Pervanadate (1 nM) at 37‚ÑÉ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.