Western blot analysis of extracts of 293T cells, using Phospho-Raptor-Ser792 antibody (TA381147) at 1:1000 dilution.293T cells were treated by Oligomycin (0.5 uM) at 37‚ÑÉ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1S.