Western blot analysis of extracts of various cell lines, using Phospho-PPP1CA-T320 antibody (TA380230) at 1:2000 dilution or PPP1CA antibody (A12468). NIH/3T3 cells were treated by Calyculin A (100 nM) at 37‚ÑÉ for 30 minutes after serum-starvation overnight. |Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 30s.