Western blot analysis of extracts of Jurkat and HeLa cells, using Phospho-PRKCQ-T538 antibody (TA380370) at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.HeLa cells were treated by serum-starvation overnight or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 30s.