Western blot analysis of extracts of Rat liver, using AMPKα2 antibody (TA380334) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

Western blot analysis of extracts of various cell lines, using AMPKα2 antibody (TA380334) at 1:500 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] AMPKα2 Rabbit pAb (TA380334) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using [KO Validated] AMPKα2 Rabbit pAb (TA380334) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.