Western blot analysis of extracts of 293T cells, using Phospho-PKC (pan) (βII Ser660) pAb (TA380357) at 1:1000 dilution or PRKCQ antibody (A5834).293T cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 90s.