Western blot analysis of extracts of THP-1 cells, using IL17A antibody (TA377426) at 1:1000 dilution.THP-1 cells were treated by PMA (80 nM) at 37‚ÑÉ for overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.