Western blot analysis of extracts of A-549 cells, using IDO1 antibody (TA377327) at 1:500 dilution.A549 cells were treated by hIFN-γ（100ng/mL） at 37℃ for 48 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.