Western blot analysis of extracts of various cell lines, using Flotillin 1 antibody (TA376280) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

Immunofluorescence analysis of NIH/3T3 cells using Flotillin 1 Rabbit pAb (TA376280) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Flotillin 1 antibody (TA376280). Western blot was performed from the immunoprecipitate using Flotillin 1 antibody (TA376280) at a dilition of 1:1000.