Western blot analysis of extracts of C6 cells, using Phospho-ABL1-Y412 pAb (TA373163) at 1:1000 dilution or ABL1 antibody (A0282).C6 cells were treated by CIP(20uL/400ul) at 37‚ÑÉ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 3s.