Western blot analysis of extracts of PC-12 cells, using Phospho-Tyrosine Hydroxylase-S40 pAb (TA382487) at 1:1000 dilution or Tyrosine Hydroxylase antibody (A0028).PC-12 cells were treated by Forskolin (30 uM) at 37‚ÑÉ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 3s.