Western blot analysis of extracts of C6 cells, using Phospho-Chk1-S345 pAb (TA374726) at 1:1000 dilution or Chk1 antibody (A7653).C6 cells were treated by ATP(5 mM) at 30‚ÑÉ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit .|Exposure time: 5min.