Western blot analysis of extracts of SH-SY5Y cells, using Phospho-EphA2-Y594 antibody (TA375927) at 1:1000 dilution. SH-SY5Y cells were treated by EphrinA1 (100 ng/mL) for 5 minutes .|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 60s.