Western blot analysis of extracts of C6 cells, using DDIT3/CHOP antibody (TA375372) at 1:1000 dilution.C6 cells were treated by tunicamycin (2 μg/ml) for 8 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.