Western blot analysis of extracts of various cell lines, using Phospho-Lamin A/C-S22 antibody (TA377944) at 1:2000 dilution or Lamin A antibody (A0249). HeLa cells were treated by Hydroxyurea (4 mM) at 37‚ÑÉ for 20 hours or treated by Paclitaxel (100 nM/mL) at 37‚ÑÉ for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37‚ÑÉ for 20 hours or treated by Paclitaxel (100 nM) at 37‚ÑÉ for 20 hours. |Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit .|Exposure time: 1s.