Western blot analysis of extracts of various cell lines, using Phospho-NPM1-T199 pAb (TA379260) at 1:1000 dilution or NPM1 antibody (A17983).HeLa cells were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours.PC-12 cells were treated by Nocodazole (1 µg/mL) at 37℃ for 16 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 10s.