Western blot analysis of extracts of 293T cells, using Phospho-PERK-T982 Rabbit pAb (TA375791) at 1:1000 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37‚ÑÉ for 5 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 90s.