Flow cytometry using the Anti-CD52 antibody CF1D12 (TA386059). Paraformaldehyde fixed Daudi cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (TA385792; isotype control, black line) or the rabbit IgG version of CF1D12 (TA386059, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.