Western blot analysis of extracts of HeLa cells, using Phospho-IKKα-S180 antibody (TA374796) at 1:1000 dilution. HeLa cells were treated by both TNF-α(20ng/mL) and Calyculin A (100nM) for 10 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 5s.