Western blot analysis of extracts of various cell lines, using Phospho-c-Jun-T93 antibody (TA377673) at 1:500 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37‚ÑÉ for 15 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37‚ÑÉ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.