Western blot analysis of extracts of 293T cells, using Phospho-c-Myc-T58 antibody (TA378818) at 1:500 dilution.293T cells were treated by Calyculin A (100 nM) at 37‚ÑÉ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.