Western blot analysis of extracts of C6 cells, using FOSL1 antibody (TA376321) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37‚ÑÉ for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.