Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Vimentin-S39 antibody (TA383241) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37‚ÑÉ for 20 hours.NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37‚ÑÉ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 1s.