Western blot analysis of extracts of various cell lines, using [KO Validated] IκBα antibody (TA379096) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 180s.

Western blot analysis of extracts of Rat liver, using [KO Validated] IκBα antibody (TA379096) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug IκBα antibody (TA379096). Western blot was performed from the immunoprecipitate using IκBα antibody (TA379096) at a dilition of 1:500.