Western blot analysis of extracts of 293T cells, using Phospho-MYH9-S1943 antibody (TA378840) at 1:2000 dilution. 293T cell lysates were treated by CIP at 37°C for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit .|Exposure time: 30s.