Western blot analysis of extracts from normal (control) and Cyclin D3 knockout (KO) HeLa cells, using Cyclin D3 antibody (TA374380) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.