Western blot analysis of extracts of various cell lines, using PKM1-specific antibody (TA379983) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 3s.

Immunofluorescence analysis of C6 cells using PKM1-specific Rabbit pAb (TA379983) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using PKM1-specific Rabbit pAb (TA379983) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.