Western blot analysis of extracts of various cell lines, using Carbonic Anhydrase 9 (CA9/G250) antibody (TA374178) at 1:100 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.