Western blot analysis of extracts of 293T cells, using Phospho-SMO-S611/615/616 antibody (TA381790) at 1:1000 dilution.293T-SMO cells were treated by SHH (0.4ug/ml) at 37‚ÑÉ after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit .|Exposure time: 1min.