Western blot analysis of extracts of various cell lines, using L1CAM antibody (TA377925) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 30s.

Immunofluorescence analysis of HeLa cells using [KO Validated] L1CAM Rabbit pAb (TA377925) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of Neuro-2a cells using [KO Validated] L1CAM Rabbit pAb (TA377925) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of SH-SY5Y cells using [KO Validated] L1CAM Rabbit pAb (TA377925) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.