Western blot analysis of extracts of various cell lines, using Phospho-Caveolin-1-Y14 Rabbit pAb (TA374309) at 1:1000 dilution.Both HeLa cells and NIH/3T3 cells were treated by Hydrogen Peroxide (2 nM) at 37‚ÑÉ for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 120s.