Western blot analysis of extracts of various cell lines, using NUCB1 antibody (TA379386) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

Immunofluorescence analysis of L929 cells using NUCB1 Polyclonal Antibody (TA379386) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using NUCB1 Polyclonal Antibody (TA379386) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.