Rabbit anti-CD3E Recombinant Monoclonal Antibody from Biorbyt

Application Notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

Application Dilutions: WB 1:1000, IP: 20 µl/mg lysate, IHC: 1:100 - 1:500, Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cell. mIF: 1:250